Design and Application of Fluorescent Aptamer Probes for Angiogenin Analysis 荧光核酸适体探针的设计及其在血管生成素分析中的应用
Angiogenin is very important physiologic function on normal tissue growth and rebuilding. furthermore, it possibilitily takes part in birthing new vein of the tissue of tumour, and acts the pathology in transferring tumour. 摘要人血管生成素对正常机体的组织发育和再生具有较重要的生理功能,同时还可能参与肿瘤组织的新生血管生成,在肿瘤转移中发挥病理作用。
The Study of the Relationship between Angiogenin and bFGF and Their Molecular Mechanisms 血管生成素与碱性成纤维生长因子之间相互作用关系及其分子机制的研究
Although the catalytic activity of angiogenin is rather weak, it is critical for its angiogenic properties. 血管生成素的核糖核酸酶活性很弱,但对它的血管生成活性是至关重要的。
Angiogenin was isolated as a tumor angiogenic factor based solely on its angiogenic activity. 血管生成素基于其促血管发生活性,最早作为肿瘤血管生成因子被分离出来。
We hypothesized abnormalities of angiogenin levels in acute coronary syndrome ( ACS), with prognostic implications for predicting adverse events. 我们假设异常的血管生成因子水平预示着急性冠脉综合症患者的不良预后。
Objective To investigate the expression of angiogenin in human hair follicle and evaluate its effect on hair growth. 目的探讨血管生成素在人毛囊中的表达及其意义。
Characterization of Nucleic Acid and Investigation of Interaction between Angiogenin and Its Aptamer Based on Atomic Force Microscopy 原子力显微镜对核酸的表征和血管生成素与核酸识体相互作用的研究
Objective To establish a simple purification technology of recombinant human angiogenin ( rhANG) for its clinical application. 目的建立表达重组人血管生长素(recombinanthumanangiogenin,rhANG)的简便纯化工艺,为扩大生产及临床应用打下基础。
The characterization of recombinant human angiogenin and its monoclonal antibody 重组人血管生长素及其单克隆抗体的特性鉴定
Methods: ELISA method was used to assay the expression and secretion of angiogenin ( ANG) after Ad · ANG transfection of BMSCs ex vivo. 方法Ad.ANG体外转染BMSCs后,通过ELISA方法检测ANG的表达和分泌情况;
Construction of angiogenin antisense eukaryotic expression vector and its effect in vitro 反义人血管生成素的真核表达载体的构建及其体外功能的初步研究
There are two modes of action when exogenous angiogenin binds to the endothelial cells. 外源ANG作用于血管内皮细胞的方式有两种:一种是通过经典的胞内信号系统;
Conclusion: The successful expression and purification of ANG-EGFP fusion protein provides materials to clarify the process of nuclear translocation of angiogenin and to explore its mechanism of action in RNA transcription. 结论:成功的表达并纯化了ANG鄄EGFP融合蛋白,利用EGFP的荧光示踪作用,该融合蛋白可用于血管生成素核转位和胞内共定位蛋白质研究。
The Recombination of Human Angiogenin Gene Fragment in Vitro 人血管生长因子基因片段的体外重组
Angiogenin ( Ang) is a single strand 14 KD alkaline protein, belonging to RNase superfamily, and which can induce the formation of newly formed vascular net. 血管生成因子(angiogenin,Ang)是一种单链的碱性蛋白质,分子量为14KD,属于核糖核酸酶超家族的一员,它重要的生物学功能是具有诱导新血管网形成的作用。
Chemical synthesis and clone of human angiogenin gene 人血管生长素基因的化学合成与克隆
The experiment demonstrated that RI might effectively block the angiogenesis that was induced by angiogenin. 实验显示RI能有效地抑制肿瘤诱导血管的生成。
As one of the ribonuclease superfamily, angiogenin ( Ang) is a protein ( 14.4KD) secreted by tumor cell and normal cell, and has very high activity to induce vascular growth. 核糖核酸酶超家族成员之一血管生成因子(Angiogenin,Ang)是肿瘤细胞和正常细胞分泌的分子量为14.4kD的蛋白质,在体内外具有强烈的诱发血管生成的活性。
Angiogenin is expressed in a wide range of different human cells. 血管生成素在人类各种细胞中均有广泛表达,可促进正常和病理条件下血管生成。
An in Vitro Nuclear Run-on Assay for Nuclear Function of Angiogenin 血管生成素对细胞核内功能的体外分析体系
The specific interaction between angiogenin and its aptamer was studied by AFM. 基于AFM力测定技术研究了血管生成素与其核酸识体(Aptamer)之间的特异性相互作用。
The single molecular pull-off force between angiogenin and its aptamer was determined using the Poisson statistical method. 采用Poisson统计的方法计算出了血管生成素与其Aptamer间的单分子相互作用力。
Construction of library of the phage genetic engineering antibody of anti-human angiogenin 抗人血管生长素噬菌体基因工程抗体库的构建
The amino acid sequence of angiogenin ( Ang) is high conservative compared with RNase A. RNaseA与血管生成因子(Angiogenin,Ang)的氨基酸有着高度保守的同源顺序。
Next we compared the binding capacity of angiogenin with rDNA in HeLa cells before and after angiogenin treatment. 随后我们进一步研究了用血管生成素处理前后HeLa细胞中血管生成素与rDNA各段的结合程度的变化。
Angiogenin plays very important roles during the process of tumor vessel and is a direct angiogenesis inducer. 血管生成素是目前为止唯一一个以促血管发生功能而命名的血管生成因子,作为促血管生成因子,在诱发肿瘤的新血管生成中起着非常重要的作用。
Angiogenin and basic fibroblast growth factor ( bFGF) are two important growth factors during angiogenesis. They can regulate proliferation, differentiation and migration of the cell, thus play important roles during the process of tumor occurrence and development. 血管生成素和碱性成纤维生长因子(Basicfibroblastgrowthfactor,bFGF)是重要的促血管生长因子,调节着细胞的增殖、分化和迁移,在肿瘤的发生发展过程中起着重要作用。
Angiogenin ( Ang) is an important factor that can initiate and promote the angiogenesis in tumor tissue effectively, and then accelerate the development and metastasis of tumor. 血管生成素(Angiogenin,Ang)是其中的重要成员之一,它能有效地起始和促进肿瘤组织血管的形成,加速肿瘤的发展和转移。
A novel simple and quick method for visualizing the real-time cellular internalization process of angiogenin was developed by using singly fluorophore-labeled aptamer probe and confocal laser scanning microscopy. 利用单荧光标记的核酸适体探针,结合激光共聚焦成像技术,对血管生成素的内化过程进行实时成像研究,发展了一种在活细胞水平上进行蛋白质分析的新方法。