Experimental studies were carried out on the fed-batch fermentation process of sorbose in a16L airlift reactor. 在全容积16L的环隙气升式反应器内,进行了高浓度山梨糖流加发酵过程的研究。
Study on the continuous fermentation of sorbitol in sorbose production 山梨醇连续发酵生产山梨糖过程的研究
This selection strongly inhibits the growth of arg-13 under stringent sorbose/ glucose condition but allows arg-13 to grow under spot test conditions. 此法在严紧山梨糖/葡萄糖条件下能强烈抑制arg-13突变株生长,但在斑点试验条件下允许arg-13突变株生长。
Three catalytic sites were predicted through bioinformatics analysis. The predicted sites of sorbose dehydrogenase were mutated by fixed point mutation method, a site was mutated into triplet codon coding three amino acids, and the activity of mutant was analyzed. 通过生物信息学分析预测出2个催化位点,通过定点突变方法对各个位点进行了突变,并对突变体做了活性分析。