Synthesis of SOPC and Its Application to Activity Assay of Tryptophanase SOPC的合成及其在色氨酸酶活力测定中的应用
Study on the Activity of Tryptophanase in Genetic Engineering Strains and Relationship between It and Expression 色氨酸酶基因工程菌的活力及其与表达关系研究
Methods: Effects of three immobilized materials, temperature, pH, monovalent canons and alcohol on the activity of tryptophanase of immobilized WW-11 were observed by enzyme assay. 方法;通过色氨酸酶活力测定,考察三种固定化材料及温度、pH、单价阳离子和乙醇对固定化WW-11色氨酸酶活力的影响。
Results: PVA served as immobilized carrier of NO. WW-11, and its activity recovery of tryptophanase was 60. 9%. 结果:以聚乙烯醇作为WW-11的固定化载体,其活力回收为60.9%。
Study on Immobilization of Tryptophanase Genetic Engineering Strain and Its Culture Conditions 色氨酸酶基因工程菌固定化及其培养条件的研究
Molecular Cloning and Expression of Tryptophanase Gene of Escherichia coli 大肠杆菌色氨酸酶基因的克隆与表达
Conclusion: The stability of tryptophanase of immobilized engineering strain by temperature and alcohol was superior to that of free strain. 结论:固定化工程菌色氨酸酶对温度、乙醇等的稳定性比游离菌有显著提高。